OUR SOLUTIONS

RNA KnockMasterX™

Enhance Gene Silencing Efficiency with the Tool for the Knockdown Prediction 

PROBLEM

Traditional RNAi design focuses almost entirely on sequence matching—but real-world gene silencing efficiency depends on much more. mRNA secondary structure, chemical modifications (like m⁶A), and target site accessibility can dramatically affect whether an siRNA or shRNA or ASO or SBO actually works. Without accounting for these critical, non-sequence features, especially when targeting pre-mRNA or noncoding RNAs,  RNAi therapies often fail or underperform, leading to wasted time, resources, and inconsistent results.

OUR SOLUTION

RNA KnockMasterX™ is a cutting-edge design tool for RNA knockdown, capable of optimizing small interfering RNA (siRNA), antisense oligonucleotide (ASO) and steric blocking oligonucleotide (SBO) designs for both coding and noncoding RNA targets. Powered by advanced AI algorithms, it delivers tailored solutions to meet your research needs. RNA KnockMasterX™ goes beyond traditional sequence-based design by integrating RNA structure, accessibility, and chemical modifications into the design process, ensuring more precise targeting. This enables greater silencing efficiency for both mRNA and noncoding RNA, making gene knockdown more reliable, effective, and accessible than ever before.

Are you interested in any of the following?

  • Designing highly efficient RNAi, antisense, or steric-blocking oligos for your target mRNA or noncoding RNA
  • Maximize gene silencing, block splicing, inhibit translation, or target structured/noncoding RNAs.

What we need from you?

  • Target RNA sequence (mRNA or noncoding RNA in FASTA format or gene/transcript ID) OR
  • Target region of interest (optional) (e.g., CDS, UTR, intron, splice site, custom region)
  • Species information (so we can use the correct transcript annotation and modification data)
  • Delivery method (optional) (e.g., siRNA, ASO, SBO—if you have a preferred chemistry or platform)
  • Any constraints or preferences (e.g., avoid SNPs, use modified bases, limit off-targets, delivery vector compatibility)

What will we deliver?

  • Ranked list of optimal target sites
  • Custom-designed oligonucleotide sequences
  • Target structure and accessibility maps
  • Off-target risk assessment
  • Publication-ready figures for grants, or manuscripts